Journal: Frontiers in Immunology
Article Title: Prognostic integration of tumor microenvironment and parthanatos-related genes in gastric cancer: a machine learning-driven risk model and immune landscape profiling
doi: 10.3389/fimmu.2026.1636331
Figure Lengend Snippet: Detection of protein expression and functional verification of CD36 and KIT. (A) Immunohistochemical staining results of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. N: Adjacent normal, T: Tumor. Brown indicates positive expression of CD36 and KIT, blue indicates cell nuclei. (B) Quantitative analysis of immunohistochemical optical density of CD36 and KIT in gastric cancer tissues and adjacent non-tumor tissues. ** denotes P < 0.01, *** denotes P < 0.001. (C) Migration status of MKN45 cells after treated with CD36 and KIT inhibitors; (D) Number of migrated MKN45 cells after treated with CD36 and KIT inhibitors * denotes P < 0.05.** denotes P < 0.01. (E) Detection of cell proliferation ability in MKN45 cells treated with CD36/KIT inhibitors * denotes P < 0.05.** denotes P < 0.01 (F) Differential analysis of cell apoptosis level in MKN45 cells treated with CD36/KIT inhibitors.*** denotes P < 0.001.
Article Snippet: Subsequently, primary antibodies against CD36 (18836-1-AP, Sanying, China) or KIT (AF6153, Affinity, USA) were added, and the sections were incubated overnight at 4°C followed by incubation with HRP-conjugated secondary antibody (K5007, Dako, Denmark) at 37 °C for 30 minutes.
Techniques: Expressing, Functional Assay, Immunohistochemical staining, Staining, Migration